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Anti-CD45 [ITS_rhCD45]-AF594

Product Type: Therapeutics (In Vivo)
Format: Anti-CD45, ITS_rhCD45, mouse variable, mouse IgG1, kappa
Clone ID: [ITS_rhCD45]
RRID: AB_2941326
Common Names: Anti-CD45 [ITS_rhCD45]-AF594 | Anti-CD45 [ITS_rhCD45]-AlexaFluor594 | ITS_rhCD45 | PR-0078
Target: CD45
Reactivity: Rhesus Macaque (Macaca mulatta)
Applications: in vivo in vivo; SIVS

The Anti-CD45 [ITS_rhCD45] antibody clone was developed for leukocyte trafficking studies using the Serial Intravascular Staining (SIVS) method (see references below). This mouse antibody clone specific to rhesus CD45 glycoprotein was produced from CHO cell lines, purified using protein A chromatographic separation, and conjugated to the Alexa Fluor dye (NHS chemistry).


SIVS Infusion protocols and research articles:


  1. Potter, E. and Roederer, M. (2022). αCD45 infusions. Bio-protocol.
  2. Anderson, K., Mayer-Barber, K., Sung, H. et al. Intravascular staining for discrimination of vascular and tissue leukocytes. Nat Protoc 9, 209–222 (2014).
  3. Potter, E., Gideon, H., Tkachev, V., Fabozzi, G., Chassiakos, A., Petrovas, C., Darrah, P., Lin, P., Foulds, K., Kean, L., Flynn, J. and Roederer, M.(2021). Measurement of leukocyte trafficking kinetics in macaques by serial intravascular staining. Science Translational Medicine 13(576). DOI: 10.1126/scitranslmed.abb4582
Modifications: AF594
Restrictions: No
Sponsor: NIAID
How to cite (Methods): The Anti-CD45 [ITS_rhCD45]-AF594 antibody was engineered and produced by the Nonhuman Primate Reagent Resource (NIH Nonhuman Primate Reagent Resource Cat#PR-0078,RRID:AB_2941326).
How to cite (Acknowledgments): The Anti-CD45 [ITS_rhCD45]-AF594 antibody used in this study was provided by the NIH Nonhuman Primate Reagent Resource (NIAID U24 AI126683).

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