The Anti-CD45 [ITS_rhCD45] antibody clone was developed for leukocyte trafficking studies using the Serial Intravascular Staining (SIVS) method (see references below). This mouse antibody clone specific to rhesus CD45 glycoprotein was produced from CHO cell lines, purified using protein A chromatographic separation, and conjugated to the Alexa Fluor dye (NHS chemistry).
SIVS Infusion protocols and research articles:
- Potter, E. and Roederer, M. (2022). αCD45 infusions. Bio-protocol. bio-protocol.org/prep1586
- Anderson, K., Mayer-Barber, K., Sung, H. et al. Intravascular staining for discrimination of vascular and tissue leukocytes. Nat Protoc 9, 209–222 (2014). https://doi-org.umassmed.idm.oclc.org/10.1038/nprot.2014.005
- Potter, E., Gideon, H., Tkachev, V., Fabozzi, G., Chassiakos, A., Petrovas, C., Darrah, P., Lin, P., Foulds, K., Kean, L., Flynn, J. and Roederer, M.(2021). Measurement of leukocyte trafficking kinetics in macaques by serial intravascular staining. Science Translational Medicine 13(576). DOI: 10.1126/scitranslmed.abb4582